Part:BBa_K3380101:Experience

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Applications of BBa_K3380101

The F30 Upstream scaffold (BBa_K3380101) was used in tandem with F30 Downstream scaffold (BBa_K3380102) flanking the iSpinach fluorescent RNA aptamer (BBa_K3380150) under the class III T7 RNA polymerase strong promoter (BBa_z0251). The constructs BBa_K3380500, BBa_K3380600 and BBa_K3380603 were synthesized to test if the iSpinach RNA aptamer would exhibit fluorescence when adding DFHBI fluorophore. The constructs were tested in optimised transcription/detection buffer (OTDB). The OTDB buffer was prepared as a 10X stock as follows: Tris base 400 mM pH 7.5 adjusted with HCl (Sigma-Aldrich, #T1503); MgCl2 6(H2O) 60 mM (Sigma-Aldrich, #M2670); DTT 100 mM (Melford, #MB1015) and Spermidine 20 mM (Alfa Aesar, #A19096.03).

User Reviews

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